Coding
Tat-lispro

Part:BBa_K1731001:Design

Designed by: Alejandro Rodriguez-Gama, Lizbeth Airais Bolaños Castro   Group: iGEM15_UNAM-CU   (2015-09-14)


Constitutive Insulin lispro


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 430


Design Notes

Many other secretion signals could be added to insulin sequence in order to take it through different pathways

Source

Synthetic, codon optimized for E. coli expression

References

[1] HoweyDC, BowsherRR, BrunelleRL, Woodworth JR: [Lys(B28), Pro(B29)]- human insulin: a rapidly absorbed analogue of human insulin. Diabetes 43:396-402,1994